Effect of Glutaraldehyde Concentration Variation on Diclofenac Sodium Scaffolds as Cross-Linking Agent

Chitosan and gelatin were used as polymer scaffolds for cartilage tissue engineering. The scaffold was a biodegradable drug delivery system diclofenac sodium to treat defects on osteoarthritis (OA). materials composed of sodium, chitosan, gelatin, cross-linking agent-glutaraldehyde (GTA) form scaffold. purpose this study investigate the effect GTA concentration variations (0.00%; 0.25%; 0.50%; 1.00%; 2.50%) characteristics release from chitosan-gelatin made by using pre-freezing method with temperature -56 ± 5°C 24 hours characterized porosity, pore size, swelling, degradation, toxicity test, released at pH body. results showed, addition increased swelling ratio 195.79 7.04% 793.49 6.92% minimized weight loss up 50.98 0.82%, percentage living cells >60%, optimal porosity 106.94 9.38 % size 135.48 89.70 µm, sustained completed in 542 following zero-order kinetic. Chitosan-gelatin is potential candidate engineering sodium.